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• This study aimed to evaluate the performance, productivity, and cost savings benefits of using an automated on-line system for the clean-up of QuEChERS extracts of food samples, including the automated preparation of calibration solutions, for pesticide residues analysis by GC-MS/MS.
• The QuEChERS extraction method, developed two decades ago, is the most common methodology applied in pesticide residues laboratories.
• However, dSPE is not always effective at removing sufficient matrix co-extractives, which can create unwanted downtime for testing laboratories.
• On-line micro solid phase extraction (μSPE) is an interesting alternative for automating the dSPE approach, resulting in cleaner extracts, reduced matrix load, improved robustness of GC-MS analysis, longer column and liner lifetimes, increased maintenance intervals, and eliminated interferences that can negatively affect pesticide identification and quantitation.

The μSPE clean-up can be fully automated, increasing productivity in pesticide residues analysis by GC-MS/MS. The study also describes an extended automated workflow including the calibration set-up.

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