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Label-Free Detection Technologies, Key Considerations, and Applications

Label-Free Detection is used for Binding Interaction Analysis. Bio-Layer Interferometry and Surface Plasmon Resonance are used for label-free biosensor binding assay methods. Biosensor Assays vs. ELISA are compared. Calorimetry and Microscale Thermophoresis are used for determining the optimal biosensor platform. Key Octet ® BLI System Attributes are highlighted. Typical Assay Workflow is discussed. Analysis on FC-Gamma Receptor Interactions in Octet ® Platforms is discussed. Selecting an assay format is discussed. Quantitation on Octet ® BLI Platforms is also discussed.

Quantification Assay for Antiviral Virus (AV) Concentration Monitoring

  • Biomolecules' interactions are key triggers for biological processes, providing targets for drug discoveries.
  • Techniques used to characterize these interactions need to mirror the level of biological complexity.
  • Label-free biosensor binding assay methods include biosensor-based Bio-Layer Interferometry (BLI), sensor chip-based Surface Plasmon Resonance (SPR), solution-based Isothermal Titration Calorimetry (ITC), and Microscale Thermophoresis (MST).
  • BLI and SPR monitor binding interactions based on molecular accumulation during complex formation.
  • BLI platforms measure light interference originating from the tip of the biosensor surface, where light wavelengths are made to reflect from two layers: a biocompatible layer at the end of the biosensor surface and an internal reference layer.
  • The interference pattern (i.e., spectral shift) in real time provides kinetics data on molecular interactions.

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