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Octet® AAVX Biosensors for Rapid and Direct Quantitation of AAV Capsids

The Octet ® AAVX Biosensor is a tool for rapid, real-time, and high-throughput measurement of AAV concentration in samples. The AAV is a popular vector in gene therapy due to its tissue specificity. Current methods to quantify virus capsid concentration, such as ELISA, are time-consuming, laborious, and have high variability. The AAVX Biosensors offer a quantitation dynamic range of 8.5x10 8 to 1.0x10 13 vp/mL, high precision, and broad AAV serotype binding specificity. The AAVX Biosensors are compatible with different samples matrices encountered in upstream and downstream process intermediates, requiring minimal sample preparation.

Octet® Biosensors for Biopharmaceutical Research

  • The AAVX Biosensors can be regenerated and re-used for up to 20 times, reducing the cost per measurement. The AAVX Biosensors can be used on all models of the Octet ® Bio-Layer Interferometry (BLI) platform except on the Octet ® N1, where further assay optimizations would be required.
  • Bio-Layer Interferometry (BLI) based on fiber optic biosensors. Yes/No binding, kinetic and affinity analysis, specific and selective detection of molecules, and relative and absolute quantitation of specific proteins.
  • Key features of the AAVX Biosensor include the ability to bind to 10 different AAV serotypes, quantitate both purified and crude viral samples, and good regenerability and re-use, which helps reduce cost per assay. The biosensors are compatible with a wide range of buffers, with the quantitation assays performed with the Octet ® Sample Diluent buffer, pH 7.4.
  • Biosensor hydration: AAVX Biosensors dipped in assay buffer for 10 minutes before use. AAV direct quantitation assay design: Initial baseline equilibration in assay buffer for 180 seconds.
  • Direct quantitation: AAVX Biosensors dipped into microplate wells for 900-1800 seconds to determine AAV vector binding rate to AAVX antibody. Biosensor regeneration for re-use: Biosensors dipped into 10 mM glycine buffer, pH 1.7 for 5-10 seconds, followed by assay buffer for 5-10 seconds.
  • Capture of AAV standard and test samples: AAV serotype standard and test samples incubated in anti-serotype-specific capture antibody coated microtiter plate.

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